منابع مشابه
Patch-clamp recording on a chip
Cristian Ionescu-Zanetti et al. have developed a technique for electrophysiological recording of whole mammalian cells on a microf luidic chip. The technique permits multiplexed recording of ion channel voltages, while leaving the cells visible for microscopic inspection and manipulation. The device records through many junctions along the microf luidic channel while a microscope observes from ...
متن کاملWhole cell patch clamp recording performed on a planar glass chip.
The state of the art technology for the study of ion channels is the patch clamp technique. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets. The most popular (whole cell) variant of the technique detects the ensemble current over the entire cell membrane. Patch clamping is still a laborious process, requiring a skilled expe...
متن کاملAutomated planar patch-clamp.
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
متن کاملWhole-Cell Patch-Clamp Recordings
The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
متن کاملOn-chip Non-invasive Voltage Clamp on Xenopus Oocytes
In order to improve the throughput of the traditional “Two Electrode Voltage Clamp” (TEVC) measurements on Xenopus oocytes, we present here a chip-based non-invasive technique for the measurement of ion channels activity. We focus especially on the integration of the electrical measurement site into a microfluidic structure, allowing rapid exchange of various small-volume solutions.
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2002
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(02)75625-7